{"ok":true,"nota":{"id":"e3433ab466dec721","slug":"logran-editar-varios-genes-a-la-vez-en-alamos-hibridos-con-una-tecnica-e3433a","titulo":"Logran editar varios genes a la vez en álamos híbridos con una técnica más eficiente","resumen":"Científicos ajustaron una herramienta de edición genética en álamos híbridos, combinando un tratamiento con calor y una modificación de la guía molecular. Apuntaron a cuatro copias de un mismo gen y lograron cambios en ambos alelos. El trabajo se publicó como preprint, sin revisión por pares.","fuente":"biorxiv","fuenteNombre":"bioRxiv · preprints","color":"#be2628","autor":"Zahn, V., Fendel, A., Fladung, M., Bruegmann, T.","url":"https://www.biorxiv.org/content/10.64898/2026.10.21.760723v1?rss=1","imagen":"/hoy/portada/e3433ab466dec721","tema":"ciencia","subtemas":["edición genética","álamos","mejoramiento forestal","crispr"],"entidades":["Populus x canescens","INRA"],"formato":"dato","entretenimiento":false,"pubTs":"2026-10-23T00:00:00.000Z","indexTs":"2026-10-23T12:08:44.567Z","hash":"9d12af43c3914dab9f55ba5d29051ed9c8488724b6ecf5c08f82036aa6660253","tsa":{"time":"Oct 23 12:09:12 2026 GMT","provider":"freetsa.org","sobre":"sello"},"stats":{"views":0,"likes":0,"clicks":0,"dwell":0},"tipo":"normal","registro":"hallazgo","porQueImporta":"Los árboles tardan años en crecer, así que mejorarlos genéticamente con rapidez es un problema real para la forestación. Esta técnica permitiría modificar varias copias de un gen en una sola vuelta, algo que antes costaba mucho tiempo. Para el sector forestal del país, eso puede acortar el camino hacia árboles más resistentes, aunque todavía falta comprobarlo fuera del laboratorio.","quienFirma":"bioRxiv","confianza":"preprint","consulta":null},"sello":{"hash":"9d12af43c3914dab9f55ba5d29051ed9c8488724b6ecf5c08f82036aa6660253","sello":"01eef23cf1fbd47fb12d1745d0810858c3cdd1bb6db67d1e7a0f4a0675ddffdf","beacon":{"drand":{"cadena":"8990e7a9aaed2ffed73dbd7092123d6f289930540d7651336225dc172e51b2ce","ronda":6577543,"aleatorio":"148ff3c2703e9e110d876f18aaf9b8635cdaed09aaaa9234294f9e3af830e57d","tiempo":"2026-10-23T12:08:30.000Z"},"nist":{"cadena":2,"pulso":1995966,"salida":"E1A403A8B4B43093D47FAE5FF75F7F008F2AC4DCB9D7B7B0B596ADEEA128C678E4DFD8A23C4DF9C82622A343AB7D201833E5BB0629B6026522BC6CE5DD15DAE0","tiempo":"2026-10-23T12:08:00.000Z"},"noAntes":"2026-10-23T12:08:30.000Z"},"verificacion":{"drand":true,"nist":true},"tsa":{"time":"Oct 23 12:09:12 2026 GMT","provider":"freetsa.org","sobre":"sello"},"original":{"titulo":"A heat-assisted ttLbCas12a workflow incorporating crRNA 3'-tailing enables efficient multiplex biallelic genome editing in hybrid poplar","descripcion":"In forest trees, high genome-editing efficiency in primary transformants is particularly important for functional genomics and trait improvement, as long generation times severely limit the use of subsequent generations to recover fully edited genotypes. This challenge is further compounded in hybrid poplar, where paralogous gene copies and allelic variation make efficient multiplex editing particularly difficult. We optimized LbCas12a-mediated editing in Populus x canescens (Populus tremula x Populus alba), clone INRA 717-1B4, by targeting four paralogous PtaABA8ox4 loci using temperature-tolerant ttLbCas12a, a two-stage heat treatment, and crRNA 3'-tailing. The heat-treatment regime preser","pubDate":"2026-10-23","url":"https://www.biorxiv.org/content/10.64898/2026.10.21.760723v1?rss=1"},"indexado":"2026-10-23T12:08:44.567Z"}}