{"ok":true,"nota":{"id":"65f9038c0637e36d","slug":"un-metodo-de-laboratorio-detecta-un-virus-que-podria-contaminar-terapi-65f903","titulo":"Un método de laboratorio detecta un virus que podría contaminar terapias génicas","resumen":"Investigadores describieron con detalle dos ensayos para detectar virus capaces de replicarse en productos de terapia génica. Uno usa cultivo celular con luciferasa y otro PCR cuantitativo sobre el gen pol. Evaluaron su sensibilidad con distintas cantidades de virus.","fuente":"biorxiv","fuenteNombre":"bioRxiv · preprints","color":"#be2628","autor":"Ebrahimiyan, H., Sayadmanesh, A., Baharvand, H., Basiri, M.","url":"https://www.biorxiv.org/content/10.64898/2026.10.27.762168v1?rss=1","imagen":"/hoy/portada/65f9038c0637e36d","tema":"ciencia","subtemas":["terapia génica","control de calidad","retrovirus","pcr","bioseguridad"],"entidades":["bioRxiv"],"formato":"dato","entretenimiento":false,"pubTs":"2026-10-29T00:00:00.000Z","indexTs":"2026-10-30T00:12:24.320Z","hash":"8db82a6c2f43eb2777a9ed4db6b4fa6000ab718fbd2e9f2f75548720138ae384","tsa":{"time":"Oct 30 00:13:11 2026 GMT","provider":"freetsa.org","sobre":"sello"},"stats":{"views":0,"likes":0,"clicks":0,"dwell":0},"tipo":"normal","registro":"hallazgo","porQueImporta":"Las terapias génicas usan virus modificados como vehículos, y los controles de calidad deben asegurar que no quede ninguno capaz de multiplicarse en el cuerpo del paciente. Este trabajo publica protocolos completos que antes faltaban, lo que permite que otros laboratorios los reproduzcan. Una prueba confiable es lo que separa un tratamiento seguro de uno que aún no se entiende del todo.","quienFirma":"bioRxiv (preprint, autores de laboratorios de terapia génica)","confianza":"preprint","consulta":null},"sello":{"hash":"8db82a6c2f43eb2777a9ed4db6b4fa6000ab718fbd2e9f2f75548720138ae384","sello":"9d7e4a5eb4ef0ce297e8b260b8775be51f765dacb92df8380ccbaba9766de920","beacon":{"drand":{"cadena":"8990e7a9aaed2ffed73dbd7092123d6f289930540d7651336225dc172e51b2ce","ronda":6596271,"aleatorio":"475c20d708c2524a0cdebe4af77dfec4e122e8884ff50a0b62e90561b365834d","tiempo":"2026-10-30T00:12:30.000Z"},"nist":{"cadena":2,"pulso":2005069,"salida":"B55044227417F6923433CBC2983B4E344B47070946A68BA8DC65AF11249229CBE5D9905DB414536D56361BCB8273863C09AB5DD19B3BEC1B620F044B5C5E59D8","tiempo":"2026-10-30T00:11:00.000Z"},"noAntes":"2026-10-30T00:12:30.000Z"},"verificacion":{"drand":true,"nist":true},"tsa":{"time":"Oct 30 00:13:11 2026 GMT","provider":"freetsa.org","sobre":"sello"},"original":{"titulo":"Implementation and Evaluation of Marker Rescue and qPCR Assays for Detection of Replication-Competent Retrovirus in Gene Therapy Products","descripcion":"Quality control of retroviral gene therapy vectors requires reliable detection of replication-competent retrovirus (RCR) particles. Cell-based assays remain the standard for detecting RCR, while PCR-based assays serve as rapid, complementary methods. Despite their widespread use, few studies provide comprehensive protocols and performance data to guide assay setup. Here we provide a detailed description and performance assessment of a cell-based firefly luciferase marker rescue assay for RCR testing. Additionally, we describe the setup and assessment of a qPCR-based RCR assay targeting retroviral pol gene sequence. Different viral copy numbers of MuLV were used to determine the final sensiti","pubDate":"2026-10-29","url":"https://www.biorxiv.org/content/10.64898/2026.10.27.762168v1?rss=1"},"indexado":"2026-10-30T00:12:24.320Z"}}