{"ok":true,"nota":{"id":"1ab59abaaf38fc2c","slug":"nuevo-metodo-acelera-la-obtencion-de-imagenes-3d-de-receptores-clave-p-1ab59a","titulo":"Nuevo método acelera la obtención de imágenes 3D de receptores clave para diseñar medicamentos","resumen":"Los científicos combinaron ingeniería de proteínas con producción celular libre de células vivas y un sistema de microfluidos automatizado. Así obtuvieron en pocos días complejos receptores listos para ser fotografiados con microscopía crioelectrónica.","fuente":"biorxiv","fuenteNombre":"bioRxiv · preprints","color":"#be2628","autor":"Guilliam, T. A., Jakimowicz, A. K., Manni, M. M., Gaber, Z. B., Paris, G., Hardwick, S. W., Cooper, L., Chirgadze, D. Y.","url":"https://www.biorxiv.org/content/10.64898/2026.10.21.760646v1?rss=1","imagen":"/hoy/portada/1ab59abaaf38fc2c","tema":"ciencia","subtemas":["receptores gpcr","microscopia crioelectronica","biologia estructural","diseno de farmacos","produccion de proteinas"],"entidades":["bioRxiv","eProtein Discovery"],"formato":"dato","entretenimiento":false,"pubTs":"2026-10-23T00:00:00.000Z","indexTs":"2026-10-23T12:03:18.526Z","hash":"6eaf023956bf8113c81c60e2eb5bd0256d0e7e22f8291540f837907b93d88989","tsa":{"time":"Oct 23 12:03:55 2026 GMT","provider":"freetsa.org","sobre":"sello"},"stats":{"views":0,"likes":0,"clicks":0,"dwell":0},"tipo":"normal","registro":"hallazgo","porQueImporta":"Muchos medicamentos actúan sobre los receptores GPCR, pero estudiarlos en 3D era lento y costoso, con procesos que tomaban semanas. Con esta técnica se pueden probar muchas variantes en paralelo y reducir el tiempo de preparación. Esto puede acortar el camino para diseñar fármacos más precisos. Es una mejora de método de laboratorio, no un tratamiento en sí.","quienFirma":"Universidad no especificada en el resumen (preprint en bioRxiv)","confianza":"preprint","consulta":null},"sello":{"hash":"6eaf023956bf8113c81c60e2eb5bd0256d0e7e22f8291540f837907b93d88989","sello":"99fdc3a59d319e233df217b3ff40fb9e2055743f132a70a615f61056b3e37419","beacon":{"drand":{"cadena":"8990e7a9aaed2ffed73dbd7092123d6f289930540d7651336225dc172e51b2ce","ronda":6577533,"aleatorio":"c4aba0dd838f5aced1ee3ce73b5a8152f120dc443db083e10a01045483b7000d","tiempo":"2026-10-23T12:03:30.000Z"},"nist":{"cadena":2,"pulso":1995961,"salida":"F41B5D0D554B9AA93D86596521336D547B043E4F52A3C280F10F60EAE511ED1743D3E56670D4E53EB431734AC5B1B8CB5DCF96D1DB26F562D058604979415129","tiempo":"2026-10-23T12:03:00.000Z"},"noAntes":"2026-10-23T12:03:30.000Z"},"verificacion":{"drand":true,"nist":true},"tsa":{"time":"Oct 23 12:03:55 2026 GMT","provider":"freetsa.org","sobre":"sello"},"original":{"titulo":"Rapid Production and Cryo-EM Structure Determination of GPCR-Nanodisc Complexes via Cell-Free Synthesis","descripcion":"G protein-coupled receptors (GPCRs) represent major therapeutic targets, yet cryo-EM structure determination of unliganded or antagonist-bound GPCRs remains severely bottlenecked by small receptor mass, detergent-induced instability, and multi-week cell-based production pipelines. Here, we combine intracellular loop 3 (ICL3) rigid fiducial engineering with a high-throughput, cell-free protein synthesis workflow to rapidly generate cryo-EM-ready GPCR-nanodisc assemblies. Using the eProtein Discovery(TM) digital microfluidics system, we performed automated nanoliter-scale screening of construct fusions and nanodisc lipid compositions, establishing that co-translational insertion into nanodiscs","pubDate":"2026-10-23","url":"https://www.biorxiv.org/content/10.64898/2026.10.21.760646v1?rss=1"},"indexado":"2026-10-23T12:03:18.526Z"}}